This book is the latest volume in a highly successful series within Comprehensive Biochemistry and provides a historical and autobiographical perspective of the development of the field through the contributions of leading individuals who reflect on their careers and their impact on biochemistry. The book is essential reading for everybody, from graduate student to professor, placing in context major advances not only in biochemical terms but in relation to historical and social developments. Readers will be delighted by the lively style and the insight into the lives and careers of leading scientists of their time.

This book is the latest volume in a highly successful series within "Comprehensive Biochemistry "and provides a historical and autobiographical perspective of the development of the field through the contributions of leading individuals who reflect on their careers and their impact on biochemistry. The book is essential reading for everybody, from graduate student to professor, placing in context major advances not only in biochemical terms but in relation to historical and social developments. Readers will be delighted by the lively style and the insight into the lives and careers of leading scientists of their time.

Miranda knows nothing of any other life but that of her own of hardship and misery. She knows nothing of the monsters that lurk in the shadows, watching and waiting silently in the darkness to prey on the weak and vunarible. She knows nothing of the life that awaits her, until she comes face to face with one such monster. Suddenly the life she knew is over and she is cast into an unbelievable and unimaginable world where human lives mean nothing.....where she is forced to kill merely to survive. Brought home to a place she doesn't know, to live amongst the monsters she never knew existed-to become one of those monsters herself. This new life brings with it a family of sorts, and within that family she is introduced to twin brothers Nathanael and Gabriel. Miranda soon realises that she has feelings for Gabriel, but he makes it all to clear that he does not reciprocate her feelings, Nathanael on the other hand seems her only ally against the contempt Gabriel shows towards her. But the brothers roles soon change when Nathanael returns home with some unexpected news, and Gabriel decides he has to tell Miranda the secret he's been keeping since her arrival at their home.

Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervous system, chemical neuroanatomy, and the measurement of neuronal metabolism in vivo and in vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABA transporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide, as well as examples of experimental data and their interpretation.